Undernutrition impairs the quality of growth plate and trabecular and cortical bones in growing rats
Acta cir. bras
;
34(3): e201900301, 2019. tab, graf
Article
in English
| LILACS
| ID: biblio-989070
ABSTRACT
Abstract Purpose:
To investigate the effects of dietary restriction on the growth plate and long bone tissue in growing rats.Methods:
Sixty male Wistar rats were randomly assigned to two groups Control (Con) and Diet-restricted (Res). After weaning, the Res rats were offered 50% of the chow ingested by the control (ad libitum food intake). The animals were subdivided into two subgroups with follow-ups up to 56 or 70 days. After euthanasia, the growth plate of tibias was analyzed by histomorphometry, micro-computed tomography, and mechanical test. The trabecular and compact bones were evaluated by histomorphometry, dual-energy X-ray absorptiometry, and micro-computed tomography (μCT). Real-time PCR was used to analyze gene expression.Results:
Although dietary restriction did not alter gene expression, several phenotypic changes were seen in the growth plate; i.e., decrease in volume, reduction in total area and height, decrease in the area ossified zones, mechanical weakening, reduction in mass of trabecular and cortical bone, lower bone density, deterioration of the trabecular and cortical microarchitecture, and trabeculae with lower collagen deposition.Conclusion:
Dietary restriction had severe detrimental effects on the growth plate and trabecular and cortical bone.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bone Density
/
Malnutrition
/
Cancellous Bone
/
Cortical Bone
/
Growth Plate
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2019
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
USP/BR
/
Universidade de Ribeirão Preto/BR
/
Universidade de São Paulo/BR
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