In vitro effect of terpenoids - rich extract of Momordica charantia on alpha glucosidase activity / Efecto in vitro del extracto rico en terpenoides de Momordica charantia sobre la actividad de la alfa-glucosidasa
Vitae (Medellín)
;
25(3): 148-153, 2018. Ilustraciones
Article
in English
| LILACS, COLNAL
| ID: biblio-995065
ABSTRACT
Background:
Momordica charantia is a vine, native to tropics and subtropics that has found use in herbal medicine. In Nigeria, the plant is locally used in the treatment of diabetes.Objective:
To investigate the inhibitory effect of terpenoid-rich extract from Momordica charantia on α-glucosidase activity.Method:
The inhibitory effect of the terpenoid-rich extract from Momordica charantia on the activity of alpha-glucosidase from Saccharomyces cerevisiae as well as the determination of the mode of inhibition were investigated using in vitro assay methods.Results:
The results revealed that the terpenoid-rich extract had a high IC50 of 1.60 mg/mL. The Lineweaver-Burk plot revealed that the extract exhibited partially mixedtype inhibition of α-glucosidase.Conclusions:
It can be inferred from this study that although terpenoid-rich extract inhibited α-glucosidase, further purification will be necessary on the extract so as to increase its potency as an inhibitor of α-glucosidase. The action of the extract on α-glucosidase provided an insight into the probable mechanism of action of Momordica charantia in reducing hyperglycemia.RESUMEN
Antecedentes Momordica charantia es una vid, nativa de las regiones tropicales y subtropicales que ha sido usada en la medicina herbal. En Nigeria, esta planta se ha usado localmente en el tratamiento de la diabetes. Objetivo:
Investigar el efecto inhibitorio del extracto rico en terpenoides de Momordica charantia sobre la actividad de la α-glucosidasa.Método:
El efecto inhibitorio del extracto rico en terpenoides de Momordica charantia sobre sobre la actividad de la α-glucosidasa y el modo de inhibición se evaluó con un ensayo in vitro.Resultados:
Los resultados mostraron que el extracto rico en terpenoides tiene un alto IC50 de 1,60 mg/mL. El gráfico Lineweaver-Burk reveló que el extracto presenta una inhibición combinada de la α-glucosidasa.Conclusión:
De este estudio se infiere que aunque el extracto rico en terpenoides inhibió la α-glucosidasa, es necesario realizar una purificación con el fin de incrementar su potencia como inhibidor de la α-glucosidasa. La acción del extracto sobre la α-glucosidasa proporciona un entendimiento del mecanismo de acción probable de Momordica charantia en la reducción de la hiperglicemia.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Terpenes
/
Momordica charantia
/
Alpha-Glucosidases
Limits:
Humans
Language:
English
Journal:
Vitae (Medellín)
Journal subject:
Pharmacy
/
Chemistry
Year:
2018
Type:
Article
Affiliation country:
Nigeria
Institution/Affiliation country:
Ahmadu Bello University/NG
/
Umaru Musa Yar'adua University/NG
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