Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
Clin. biomed. res
;
34(2): 64-68, 2014. graf, tab
Article
in English
| LILACS
| ID: biblio-997748
ABSTRACT
Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure
Full text:
Available
Index:
LILACS (Americas)
Main subject:
RNA, Viral
/
Hepatitis C
/
Hepacivirus
/
Untranslated Regions
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Clin. biomed. res
Journal subject:
Medicine
Year:
2014
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Estadual de Produção e Pesquisa em Saúde/BR
/
Hospital de Clínicas de Porto Alegre/BR
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