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Assessment of the oxidative metabolism of calves' bronchoalveolar cells: comparison between NBT and fluorimetric techniques / Mensuração do metabolismo oxidativo de células broncoalveolares de bezerros: comparação entre as técnicas de NBT e fluorimétrica
Artner, Bruna; Reck, Angela; Coelho, Alessandra Mayer; Prediger, Julio José; Pontarolo, Desiree Vera; Bertagnon, Heloisa Godoi.
  • Artner, Bruna; State University of the Central-West. US
  • Reck, Angela; State University of the Central-West. US
  • Coelho, Alessandra Mayer; State University of the Central-West. US
  • Prediger, Julio José; State University of the Central-West. US
  • Pontarolo, Desiree Vera; State University of the Central-West. US
  • Bertagnon, Heloisa Godoi; State University of the Central-West. US
Arq. Inst. Biol ; 85: e0972018, 2018. ilus, tab
Article in English | LILACS, VETINDEX | ID: biblio-999165
ABSTRACT
Evaluation of alveolar macrophage functions of cattle is an important tool in order to assess whether measures taken during the cattle husbandry can decrease the respiratory tract defense. The aim of this study was to determine whether dexamethasone used at therapeutic dose can affect the oxidative metabolism of alveolar macrophages of cattle. This was evaluated by two tests, the fluorometric and colorimetric. The similarity of the results was studied, using alveolar macrophages of six healthy cattle, obtained from bronchoalveolar lavage on a basal and an immunosuppressant moment after the application of dexamethasone. For the fluorometric test, alveolar macrophages were incubated with Staphylococcus aureus and 2'-7'dichlorohidroflurescein, and analyzed by flow cytometer. For the colorimetric test, alveolar macrophages were incubated with Phorbol 12- miristate-13 acetate and nitroblue tetrazolium, dissolved and analyzed in a spectrophotometer. It was noted that dexamethasone therapeutic dose (0.05 mg/kg) reduced the functions of alveolar macrophages from healthy bovine. This result was observed by both tests with the difference that the flow cytometry assay was more informative for identifying which specific cellular function has been compromised.(AU)
RESUMO
A avaliação das funções de macrófagos alveolares de bovinos é uma ferramenta importante no intuito de analisar se medidas adotadas durante a criação desses animais diminuem a defesa do trato respiratório, aumentando a susceptibilidade à pneumonia. O objetivo deste trabalho foi verificar se a dexametasona em dose terapêutica afeta o metabolismo oxidativo de macrófagos alveolares de bovinos por meio de duas provas, a fluorimétrica e a colorimétrica, avaliando a similaridade dos resultados. Para tanto, utilizaram-se macrófagos alveolares de seis bovinos sadios, obtidos por meio de lavados broncoalveolares em um momento basal e em um momento imunossupressor, após a aplicação da dexametasona. Na prova fluorimétrica, os macrófagos alveolares foram incubados com Staphylococcus aureus e o fluorófilo 2'-7' diclorohidrofluresceína e analisados por citometria de fluxo. Na prova colorimétrica, os macrófagos alveolares foram incubados com Phorbol 12- miristate-13 acetate e nitroazul de tetrazolium, dissolvidos e analisados em espectofotômetro. Observou-se que a dexametasona em dose terapêutica (0,05 mg/kg) reduziu as funções dos macrófagos alveolares de bovinos sadios, sendo este resultado obtido em ambas as provas, com a diferença de que o teste de citometria de fluxo foi mais esclarecedor por identificar qual função celular específica foi comprometida.(AU)
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Respiratory System / Macrophages, Alveolar Limits: Animals Language: English Journal: Arq. Inst. Biol Year: 2018 Type: Article Institution/Affiliation country: State University of the Central-West/US

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Full text: Available Index: LILACS (Americas) Main subject: Respiratory System / Macrophages, Alveolar Limits: Animals Language: English Journal: Arq. Inst. Biol Year: 2018 Type: Article Institution/Affiliation country: State University of the Central-West/US