Inhibitory effect of Trigonella foenum-graecum on galactose induced cataracts in a rat model; in vitro and in vivo studies

ABSTRACT

To evaluate the in vitro and in vivo anti-cataract potential of Trigonella foenum-graecum [TF] on galactose induced cataracts in an animal model. In the in vitro group, enucleated rat lenses were maintained in organ culture containing Dulbecco's Modified Eagles Medium alone [normal group], or with the addition of 30 mM galactose [control group]. The medium in the test group was supplemented with both galactose and TF. All lenses were incubated at 37°C for 24 hours and then processed for determination of levels of reduced glutathione and malondialdehyde. In the in vivo group, cataracts were induced in rats by a 30% galactose diet alone [control] or with the addition of TF [treated group]. Reduction [26%] in glutathione level and elevation [31%] in malondialdehyde content were observed in controls as compared to normal lenses. TF significantly [P<0.01] restored glutathione and reduced malondialdehyde levels as compared to controls. A significant delay in the onset and progression of cataract was observed with 2.5% TF diet; after 30 days none of the treated eyes developed mature cataracts as compared to 100% of control eyes. TF can delay the onset and progression of cataracts in an experimental rat model of galactose induced cataracts both in vitro and in vivo