New Egyptian Journal of Medicine [The]. 2008; 38 (6 Supp.): 32-39
in English
| IMEMR
| ID: emr-101454
ABSTRACT
The identification of susceptible from refractory Biomphalaria alexandrina snails to Schistosoma mansoni is important in transmission studies and control programs. In this study, a trial was made in this respect utilizing three techniques, namely protein electrophoresis, assay for hepatic enzymes activities and Random Amplification Polymorphic DNA [RAPD]. The results show that, in protein electrophoresis there is one shard band only between susceptible and refractory snails [68.41KD]. The activities of hepatic enzymes, aspartate aminotransferase [AST] and alanine aminotransferase [ALT], alkaline [ALKP] and acid phosphatase [AcP], were higher in the tissue homogenate and hemolymph of susceptible snails than refractory ones. In RAPD, utilizing OPA-9 primer, approximately 1,900 bp band was characteristic for susceptible snails but did not appear in refractory ones
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Schistosoma mansoni
/
Snails
/
Blood Protein Electrophoresis
/
Schistosomiasis mansoni
/
Random Amplified Polymorphic DNA Technique
/
Disease Susceptibility
/
Liver
Type of study:
Controlled clinical trial
Language:
English
Journal:
New Egypt. J. Med.
Year:
2008
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