Potential protective role of vitamins C and E administration on renal and retinal toxicity induced by organophosphorus compound chloropyrifos

ABSTRACT

The potential protective role of combined administration of vitamins C and E was sought to ameliorate the toxicity of chloropyrifos [organophosphorus compound]. Biochemical and histological effects were studied on blood, kidney and retina of rats. 40 Wistar strain rats were divided into 4 groups. Group I was the control group which received an equivalent volume of saline i.m and i.p daily for 7 days; group 11 was the model of toxicity which was induced by an oral administration of a single dose of 63mg/kg chloropyrifos dissolved in corn oil; group III was the model of toxicity treated daily for 7 days a single I.M. injection of vitamin C and I.P injection vitamin E and group IV was the positive control which received the vitamins without any toxicity induction, blood samples were taken at the 7[th] day of experiment, then erythrocytes reduced glutathione [GSH], serum malondialdehyde [MDA] and soluble FAS ligand [sFASL] concentrations were tested. The animals were then sacrificed and specimens of kidneys and retinas were collected and processed for paraffin sections and semithin sections to be examined by light microscope. The biochemical parameters showed significant improvement after vitamin C and E administration also the histological pictures of kidneys and retinas were improved as compared to the toxicity model rats. Combined administration of vitamins C and E can reduce oxidation, apoptosis and histopathological changes of kidney and retina induced by organophosphorus compound chloropyrifos toxicity