[Polyclonal antibody production against bovine serum albumin conjugated artemisinin in rabbit]
IJVR-International Journal of Veterinary Research. 2008; 2 (1): 87-94
in English
| IMEMR
| ID: emr-101828
ABSTRACT
The aim of the present study was to produce a polyclonal antibody against bovine serum albumin [BSA] conjugated with artemisinin. To gain an immunogenic character of artemisinin, a carboxyl group was added to it using mixed anhydride method. Then, the reactive compound of artemisinin was conjugated with BSA. The BSA+artemisinin were injected to white female New Zealand rabbits for two times. In the first injection, the Fraud's complete adjuvant was used, and two weeks later, a booster injection was carried out with a Fraud's incomplete adjuvant. Two weeks later, blood samples were collected; their serum were separated and frozen until assessment. The production of antibody against BSA+artemisinin was assayed by Immunoblot technique. Antibody was separated and concentrated by saturated ammonium sulfate. The assay confirmed the successful production of an antibody against BSA+artemisinin as a fundamental step in investigation of pharmacodynamics and pharmacokinetics of artemisinin
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Rabbits
/
Serum Albumin, Bovine
/
Artemisia
/
Antibody Formation
Limits:
Animals
Language:
English
Journal:
Int. J. Vet. Res.
Year:
2008
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