[Characterization of molecular and biochemical properties of some metastatic breast cancer cells]

ABSTRACT

Study of the molecular and biochemical properties of gene expression, of the certain breast cancer cell lines metastatic [e.g. HTB-30 SK-BR-3 and HTB-21 CAMA-1]; non-metastatic [e.g. CRL-2315 HCC70 and HTB-126 HS578T]; estrogen receptor positive [ER[+]; e.g. HTB-22 MCF7 and CRL-2329 HCC1500] and estrogen receptor negative [ER-; CRL2335 HCC1806, HTB-132 MB468] was carried out. After all the breast cancer cell lines, in an appropriate cell propagation medium, have been cultured until enough cells were generated [as controls], and treated with one of the fatty acids omega-3 or omega-6 [as samples of treated cells], cancer cells were isolated, then mRNA of these cells were extracted. Microarray technique was applied for studying the genetic sites of the breast cancer cells, and detection the sites where the fatty acids are affected. Confirmation of the respective results have been undertaken using Real Time Polymerase chain Reaction [Real Time PCR] [RT-PCR]. Data resulted from microarray analysis showed many genes that are involved in signaling, lymphocyte activation, apoptosis, cellular growth and proliferation. Also, the results detect an obvious difference between effect of the two studied fatty acids. Omega-3 [Eicosapentaenoic acid "EPA"] works as up regulator of the ER-breast cancer cell lines, while the omega-6 [arachidonic acid "AA"] works as up regulator of the ER+ breast cancer cell lines. This study explored the importance of the ER status in how breast cancer cells respond to omega-3 and omega-6 fatty acids, and the identification of key biomarkers that may shed light on the differential effects of these fatty acids on breast cancer cell lines. This research collaboration has set the stage for future studies to correlate genetic modification of the ER with the cell response to fatty acids, which may have a future applications in the therapeutic managements