[Effect of bonding agent on sealant microleakage in saliva-contaminated enamel]

Many clinical studies have reported on the success of sealants with respect to caries reduction. Saliva contamination during the procedure is the main reason in sealant's failure. The aim of this study is to evaluate the effect of two types of adhesive [Excite, Prompt-L-Pop] on reducing sealant microleakage in saliva-contaminated enamel. In this experimental study, 40 intact premolar teeth were chosen and divided into four groups. Group 1: the teeth were etched and sealed with sealant without any contamination. Groups 2 and 3: The teeth were etched and exposed to saliva for 10 seconds, and air-dried. Consequently, a bonding agent [Excite, Prompt-L-Pop] was applied prior to the sealant application and curing. Group 4: After being etched and contaminated, the teeth were sealed without any bonding agent. After some processes the teeth were bucolingualy sectioned and the samples were evaluated under stereomicroscope. The findings were statistically analyzed, using Kruskal-Wallis and Mann-Whitney tests. The results showed that the amount of microleakage in the saliva contaminated fissure sealant without bonding agent group had a significant difference with the sealant without saliva contamination group [p <0.05]. Besides, there was no significant difference in the amount of microleakage between Excite, Prompt-L-Pop groups and the non-contaminated fissure sealant group. Exposing the enamel to the saliva after acid etching and prior to sealant application would increase the microleakage significantly. Using Excite and Prompt-L-Pop, the dentin adhesives, after salivary contamination would decrease the microleakage to the extent of non-salivary contaminated fissure sealant group