Mediterranean glucose-6-phosphate dehydrogenase [G6PD[c563t]] mutation among jordanian females with acute hemolytic crisis

ABSTRACT

To evaluate the G6PD[c563t] Mediterranean mutation among Jordanian females who were admitted to Princess Rahma Teaching Hospital [PRTH] with/or previous history of favism. A descriptive study. Jordanian University of Science and Technology and PRTH, from October 2003 to October 2004. After obtaining approval from the Ethics Committee of Jordanian University of Science and Technology, a total of 32 females were included in this study. Samples from 15 healthy individual females were used as a negative control. Blood samples from these patients were collected and analyzed by allele-specific polymerase chain reaction [AS-PCR] to determine the GGPDcsesr mutation. Twenty one out of 32 patients were found to be G6PD[C563t] Mediterranean mutation [65.6%] positive. Three out of 21 patients were homozygous and remaining 18 were heterozygous for G6PD[C563t] Mediterranean mutation. Eleven [34.4%] out of 32 patients were found to be negative for G6PD[C563t] mutation indicating the presence of other G6PD mutations in the study sample. G6PDC563T Mediterranean mutation accounted for 65.6% of the study sample with favism in the North of Jordan. There is likely to be another G6PD deficiency variant implicated in acute hemolytic crisis [favism]