Proteome analysis of mouse brain exposed to chronic hypoxia

ABSTRACT

Chronic hypoxia exists in many diseases, including cancer. The subject of our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain. Using the emPAl protocol, we performed a quantitative proteomic approach to characterize the global proteome in the mouse brain exposed to 7% O[2] for 48 hours. Utilizing the emPAl protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression. Deregulated proteins were mainly involved in cell metabolism, apoptosis, Ca[2+] signaling, pentose phosphate pathway, 14-3-3 protein mediated signaling cascades and protein degradation. The obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia