Influence of bovine serum albumin on the antibacterial activity of endodontic irrigants against enterococcus faecalis

ABSTRACT

It has been demonstrated that organic content of the root canals can influence the antimicrobial capability of chemical irrigants. The aim of this study was to evaluate the effect of bovine serum albumin [BSA], as an organic material, on the antimicrobial activity of several intracanal irrigants. Bactericidal activity of Ethylenediaminetetraacetic acid [EDTA] 17%, citric acid 10%, Sodium hypochlorite [NaOCl] 5.25%, Chlorhexidine 0.2% [CHX], Smear Clear and Cetrimide 0.5% were tested by means of dilution-neutralization method. Contact times were 10 and 30 seconds, 5, 10, 30, 60 minutes and 24 hours. First 950 lamda of the medicament was mixed with 50 lamda of the bacterial suspension in an Eppendorf test tube. The suspensions were thoroughly mixed. Sterile water served as negative controls. After each contact time, 100 lamda of samples was transferred to the Eppendorf test tubes which contained neutralizers. After 5 minutes, 50 lamda of serial dilutions were cultured on brain heart infusion agar and incubated in aerobic conditions. Then colonies were counted and reported as cfu/mL. In half of the samples, medicaments were suspended in BSA 0.5% 30 minutes before examination to assess its possible inhibitory effect on the antibacterial activity. NaOCl 5.25%, Cetrimide 0.5% and Smear Clear showed bactericidal activity within seconds after the incubation. BSA had no inhibitory effect on bactericidal activity of these three medicaments. CHX took 5 and10 minutes to kill all bacterial cells in the absence and presence of BSA, respectively. Citric acid and EDTA showed the least antibacterial activity. In the conditions of this study, NaOCl 5.25%, Cetrimide 0.5% and Smear Clear were significantly more effective against E. faecalis than EDTA 17% and citric acid 10% in the presence and absence of BSA. Also, in the presence of BSA, bactericidal activity of CHX 0.2% against E. faecalis was significantly more than EDTA after 10 and 30 minutes of contact time. EDTA and citric acid showed the least bactericidal activity