In vitro culture of Helicobacter pylori: comparative analysis of different media and sensitivity testing
Infectious Diseases Journal of Pakistan. 2003; 12 (2): 43-44
in English
| IMEMR
| ID: emr-104495
ABSTRACT
100 gastric biopsies, in two transport media, were homogenized and cultured on three media under microaerophilic conditions. Columbia agar with commercial antibiotic supplement [containing 10 mg Vancomycin, 05mg trimethoprim, 05 mg cefsulodin and 05 mg ampotericin B per litre] yielded the best results. Growth was obtained in 3-7 days. The resistance to nalidixic acid and rapid urease production of H. pylon could be used to differentiate this species form cainpylobacter spp. Metronidazole resistance was found in 27/43[63%] of isolates. All the isolates were found to be sensitive to Amoxycillin, Clarithromycin and Tetracycline
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Trimethoprim
/
Drug Resistance, Microbial
/
Vancomycin
/
Nalidixic Acid
/
Cefsulodin
/
Amphotericin B
/
Clarithromycin
/
Culture Techniques
/
Amoxicillin
/
Metronidazole
Language:
English
Journal:
Infect. Dis. J. Pak.
Year:
2003
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