Research of glycosylated granulocyte colony-stimulating factor on mobilizing cell regeneration of bone marrow stem cells and repairing injured myocardium

ABSTRACT

To compare the differences between glycosylated and non-glycosylated granulocyte colony-stimulating factor [G-CSF] on mobilizing cell regeneration of bone marrow stem cells and repairing injured myocardium. In the acute myocardial ischemia model, 40 Sprague-Dawley rats [8 weeks old, weight 200-250 g] were successfully established with isoproteronol [ISO] and randomly and evenly divided into 4 groups Group G [injected with glycosylated G-CSF], Group N [non-glycosylated G-CSF], Group C [[control] normal saline for 7 days], and Group M [model]. At day 5, all rats were labeled with bromodeoxyuridine [BrdU]. At day 28, the proportion of CD34+ cells, myocardial BrdU immunohistochemical stain, myocardial capillary density, myocardial fibrosis and cardiac function was detected among the 3 groups. The study was carried out at the Central Laboratory, Tongji Hospital of Tongji University, Shanghai, China between 1 July 2009 and 31 July 2009. In Group G, the proportion of CD34+ cells and cardiac function was significantly higher than groups N and C, and BrdU positive cells in myocardium were higher than those other groups. Diaminobenzidine stained positive cells in striated muscle tissue possessed more cardiocyte-like structure, higher myocardial capillary density, and less myocardial fibrosis in group G compared with other 2 groups. Glycosytated G-CSF might possess stronger capability to mobilize cell regeneration of bone marrow stem cells and repair injured myocardium