Effects of skimmianine, a quinoline alkaloid of aegle marmelos correa roots, on the histamine release from rat mast cells

ABSTRACT

Skimmianine is a quinoline alkaloid isolated from the roots of Aegle marmelos Correa. In the study, we studied the effects of skimmianine on the histamine release from rat mast cells. The study was performed by using two cell lines, rat basophilic leukemia [RBL-2H3] cell line, and rat peritoneal mast cells [RPMCs]. DNP[24]-BSA, thapsigargin, ionomycin, compound 48/80 and PMA were used as inducers for histamine release from rat mast cell. Skimmianine markedly inhibited the histamine release from RBL-2H3 cells induced by DNP[24]-BSA, thapsigargin and ionomycin. The effect suggested is related to Ca[2+] signaling since skimmianine showed strong effects when the histamine release induced by Ca[2+] signal stimulants [thapsigargin and ionomycin]. It is supported that skimmianine altered the influx of [45] Ca[2+] into the cells. In RPMCs experiment, skimmianine also suppressed the histamine release induced by Ca[2+] stimulants, and phorbol myristate acetate [PMA]. However, skimmianine had no effect on the histamine release induced by compound 48/80. Based on the results, the inhibitory effects of skimmianine on the histamine release from mast cells might involve some mechanisms related to intracellular Ca[2+] signaling events and protein kinase C signaling possessing a main role in granule exocytotic processes