[Frequency and importance of jak2v617f mutation among Iranian patients with polycythemia vera, essential thrombocythemia and primary myelofibrosis]
Medical Sciences Journal of Islamic Azad University. 2010; 20 (1): 29-35
in Persian
| IMEMR
| ID: emr-105434
ABSTRACT
Detection of JAK2V617F mutation was widely used in the diagnosis and classification of myeloproliferative neoplasms. In this study, frequency of JAK2V617F mutation among Iranian patients with polycythemia vera [PV], essential thrombocythemia [ET] and primary myelofibrosis [PMF] was studied. In this basic study, blood samples of 174 patients with polycythemia vera [n=57], essential thrombocythemia [n=84] and primary myelofibrosis [n=33] were evaluated for JAK2V617F mutation. Genomic DNA was extracted from peripheral blood. After quality control of extracted DNA, the JAK2-V617F mutation was analyzed using allele-specific PCR. All PCR products were analyzed by polyacrylamide gel [5%] electrophoresis and silver staining. One hundred and eleven out of 174 patients [63.8%] were positive for the presence of the JAK2V617F mutation. Frequency of mutation was 82% [47/57] in PV, 57% [48/84] in ET and 48% [16/33] in PMF. This study showed that detection of JAK2-V617F mutation using allele-specific PCR lead to better diagnosis and treatment of Iranian patients with different MPNs
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Polycythemia Vera
/
Alleles
/
Primary Myelofibrosis
/
Thrombocythemia, Essential
/
Mutation
/
Myeloproliferative Disorders
Limits:
Humans
Language:
Persian
Journal:
Med. Sci. J. Islam. Azad Univ.
Year:
2010
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