Effect of renal ischemia / reperfusion [I/R] injury on glomerular filtretion rate [GFR] and tubular functions

ABSTRACT

Renal ischemia reperfusion [I/R] injury is of great clinical interest because of its role in renal failure, renal graft rejection and potential future chronic renal complications. Ten mongrel dogs were the material of the present study. Animals were subjected to renal ischemia by clamping the left renal artery for 30 minutes. After releasing the artery clamp six clearance periods [10 min each] were allowed to determine in each period glomerular filtration rate [GFR] by determining the inulin clearance [C[IN]], urinary flow rate [v], urinary excretion rate [UE] for Na[+], K[+], Cl[-],% Fractional excretion of sodium [%FE[Na]+], for both the ischemic left kidney and contralateral control kidney. The ischemic / control ratio [I/C] was determined for all the above parameters determined. v, UE[K+] decreased during the first collection period after reperfusion [10 min]. v increase markedly after that. GFR [C[IN]] and UE[K+] decreased also during the remaining 5 collection periods [50 min]. UE[Na+], UE[CI-] and FE[Na]% increased dramatically allover the collection periods [60 min]. The mechanisms of these changes were discussed. Gradual renal reperfusion and pre and post administration of antioxidants and anti-inflammatory drugs are recommended to ameliorate the deleterious affects ofl/R on the structure and function of the kidney