Genetic analysis of Blastocystis hominis isolated from symptomatic and asymptomatic human hosts in Egypt
Journal of the Egyptian Society of Parasitology. 2009; 39 (1): 99-109
in English
| IMEMR
| ID: emr-105962
ABSTRACT
Extensive genomic polymorphism was demonstrated among morphologically identical B. hominis isolates. A genetic diversity would be a powerful tool for identification or classification of B. hominis subtypes. In this study, 14 Egyptian B. hominis isolates were collected, 5 of them were isolated from asymptomatic people whose infections were detected during routine medical check-up and 9 were isolated from patients with gastrointestinal symptoms. Restriction fragment length polymorphism [RFLP] analysis of PCR amplified small-subunit rDNA [SSU rDNA] was used to study genetic diversity of B. hominis isolates by 3 different restriction enzymes [Hin-fl, Rsal and Sau3AI]. Cluster analysis of the riboprint patterns showed 7 distinct genotypes out of 14 B. hominis isolates, 4 were previously reported riboprints and 3 were new ones. The frequency of intestinal symptoms was 64% in Blastocystis cases, Abdominal pain was the most frequent symptom 78% [7/9]. There was no definite correlation between RFLP-banding pattern or genetically distinct genotypes and pathogenecity
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Signs and Symptoms
/
Genetic Variation
/
Polymorphism, Restriction Fragment Length
/
Polymerase Chain Reaction
/
Genotype
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
J. Egypt. Soc. Parasitol.
Year:
2009
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