Multilocus genetic analysis of cryptosporidium isolates from Saudi Arabia
Journal of the Egyptian Society of Parasitology. 2008; 38 (2): 645-658
in English
| IMEMR
| ID: emr-106009
ABSTRACT
A total of 35 Cryptosporidium positive samples were collected from children in Jeddah city. The samples were microscopically examined with Ziehl Neelsen [ZN] and Auramin phenol [AP] staining methods. Cryptosporidium antigen was detected in the faecal samples by using the Cryptosporidium ELISA kit. Cryptosporidium sp. were identified by targeting an 840 bp of the hyper-variable region of the 18S rRNA gene and about 550 of the first domain [N terminal] of the COWP gene. The subgenotypic identification of C. parvum and C. hominis isolates was done by targeting the sporozoite antigen gp 15/45 760 gene. Four sp. were identified; C. hominis 13/35 [37%], C. parvum 15/35 [42.9%], C. meleagridis 1/35[2.9%] and C. muris 1/35 [2.9%]. One isolate was a mixed infection of C. parvum and C. hominis
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Polymorphism, Restriction Fragment Length
/
Child
/
Polymerase Chain Reaction
/
Feces
/
Genotype
/
Antigens, Protozoan
Language:
English
Journal:
J. Egypt. Soc. Parasitol.
Year:
2008
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