Identification of digestive hydrolases in the larval midgut of the camel nasal bot fly cephalopina titilla tor clark [diptera: Oestridae]
Journal of the Egyptian Society of Parasitology. 2007; 37 (2): 541-556
in English
| IMEMR
| ID: emr-106027
ABSTRACT
Activities of digestive hydrolases associated with midgut of the third instar larva of Cephalopina titillator were investigated. Based on the hydrolysis of synthetic substrates and optimum pH, it was found that C. titillator midgut contains trypsin-like [optimum pH, 9], chymotrypsin esterase-like [optimum pH, 8], carboxypeptidase A and B [optimum pH at 8.5 and 7 respectively], alkaline- and acidphosphatase [optimum pH at 9 and 5 respectively] and membrane bound leucine aminopeptidase [optimum pH, 8]. An acid proteinase activity was detected, by the ability to hydrolyze acid denaturated haemoglobin; and it seems to be close to pepsin than cathepsin-like enzyme. It has a maximum activity at pH 3.5. alpha-Glucosidase activity, and was also identified [optimum pH at 6] in the midgut, and seems to be membrane bound
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Peptide Hydrolases
/
Acid Phosphatase
/
Alkaline Phosphatase
/
Aminopeptidases
/
Hydrogen-Ion Concentration
/
Hydrolases
/
Larva
/
Nasal Cavity
Limits:
Animals
Language:
English
Journal:
J. Egypt. Soc. Parasitol.
Year:
2007
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