Immunophenotyping and genotyping lymphoproliferative lesions of the skin

ABSTRACT

Various samples from lymphoproliferative diseases in the skin were analyzed by PCR with probes of T cell receptor. Phenotyping was done on frozen sections applying monoclonal antibodies for LCA, CD2, CD3, CD4, CD5, CD7, CD8, CD15, CD20, CD30, and Ki67. The panel for paraffin-embedded material includes monoclonal antibodies for LCA, CD45RO, CD43, CD20 and CD15. It was concluded that diagnostic criteria of MF were based on CD4+ and CD8- with negativity of CD7 +/- CD5. Therefore, phenotyping is helpful in the following conditions Establish malignant criteria in some early lymphoproliferative lesions and help to rule out malignancy in benign cellular lesions, confirm malignant criteria in clinicopathologic diagnosed cases and help to differentiate between T and B cell neoplasms