beta-lactamase typing by substrate hydrolysis in clinical isolates of methicillin resistant Staphylococcus aureus
Iranian Journal of Clinical Infectious Diseases. 2009; 4 (4): 202-206
in English
| IMEMR
| ID: emr-106518
ABSTRACT
Staphylococcus aureus is one of the most important causes of nosocomial infections and most clinical isolates are multidrug resistant. Resistance to beta-lactam antibiotics is most often due to bacterial beta-lactamase production. Characterization of beta-lactamases is important for choosing appropriate antibiotic therapy. Thirty methicillin resistant Staphylococcus aureus [MRSA] were identified by standard biochemical methods. Antibacterial susceptibility to 9 beta-lactam antibiotics was determined. Beta-lactamase production was shown in all isolates using the colony iodometric test and nitrocefin discs. Beta-lactamase typing was carried out by measuring the relative substrate hydrolysis rates. The MRSA isolates were resistant to the majority of beta-lactam antibiotics. The results showed that 90% of the isolates displayed type A substrate hydrolysis profile of beta-lactamase. The alarming high level of resistance to beta-lactam antibiotics including methicillin and 3[rd] generation beta-lactams show the need for extensive studies on alternative treatment protocols and use of new drugs
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Staphylococcus aureus
/
Beta-Lactamases
/
Drug Resistance, Microbial
/
Microbial Sensitivity Tests
/
Cross Infection
/
Bacterial Typing Techniques
Type of study:
Practice guideline
Language:
English
Journal:
Iran. J. Clin. Infect. Dis.
Year:
2009
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