Effect of environmental conditions on the production of polygalacturonase by the edible mushroom [volvariella volvaceae]

Effect of environmental conditions including temperature, pH, shaking rate, ratio of medium to flask volume and inoculum percentage was studied to optimize conditions for the production of polygalacturonase by Volvariella volvaceae. No enzyme production was detected at 39C or when the fungus was grown under static conditions. The polygalacturonase enzyme was optimally produced at 34C, pH 4.5, 1% inoculum and 100 rpm shaking rate using two production media. The ratios between the medium to flask volume were 1: 3.3 and 1: 5 when the production medium contained 0.0008% CoCl2 or 0.525% MgSO4.7H2O, respectively