Optimization of different cultural conditions for m-cresol degradation by free and immobilized bacillus laterosporus cells
Bulletin of High Institute of Public Health [The]. 1995; 25 (4): 991-1006
in English
| IMEMR
| ID: emr-107106
ABSTRACT
Optimization of the cultural conditions that facilitate the degradation of m-cresol by a locally isolated bacterial strain Bacillus laterosporus free and entrapped cells was attempted. Bacterial cells entrapped in 2% Ca-allginate beads were more active than free cells and showed a 20% higher rate of m-cresol degradation. Medium No. 1 was most favorable for m-cresol utilization. Supplementing the medium with yeast extract stimulated degradation especially at concentration 0.2 g/l. Optimum concentration of ammonium salts, phosphate salts and magnesium sulfate were 3 g/l and 1.5 g/l, respectively. Optimum concentration of trace elements was 4 ml/l and its omission reduced the rate of degradation. Initial pH of the medium that gave the highest rate of degradation was pH 7 and incubation temperature of 35C. Addition of some amino acids to the mineral medium did not improve degradation rate. The best alginate concentration was 2% and the optimum quantity of beads was 20 ml/ 100 ml medium. The rate of degradation increased by increasing the m- cresol concentration up to 500 mg/l, while higher concentrations [1 g/l] decreased the degradation ability of the entrapped cells
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Bacillus
/
Biodegradation, Environmental
/
Cresols
Language:
English
Journal:
Bull. High Inst. Public Health
Year:
1995
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