Effect of cephalosporin, kanamycin and irradiation on total lipids, phospholipids, neutral lipids and sterols contents of bacillus megaterium and E. coli

ABSTRACT

Cephalosporin treatment decreased total, phospho and neutral lipids of B. Megaterium and E. Coli in both solid and liquid media, expect neutral lipids of E. coli treated with cephalosporin. Total neutral and sterols contents of B. megaterium treated with kanamycin decreased by using solid media and increased in liquid media, while phospholipids increased when both media are used. On the other hand, all lipid fractions of E. coli treated with kanamycin increased, when both solid and liquid media were used. Qualitative and quantitative variations were noticed in the fatty acids pool of the non-treated and treated B. Megaterium and E. coli. In the treated inocula, one or more fatty acids disappeared from the treated cells. Percentage of the saturated fatty acids of control and treated B. Megaterium were less than E. Coli. A significant increase in total, neutral lipids and sterols contents were recorded in cells of B. Megaterium arising from irradiated inocula, while there was a significant decrease in the phospholipids contents of irradiated inocula. On the other hand, E. coli behaved in opposite direction of B. Megaterium. Radiation increased the percentage of saturated fatty acids of both organisms than control