effects of triton X-100 [nonethylene glycol octylphenol ether] on the osteoninduction capacity of the demineralized bone powder [experimental study]

ABSTRACT

The present study tested the hypothesis that Triton X-100 [non-ethylene glycol octylphenol ether] as a detergent would expose the host cells to the demineralized bone powder [DBP] by dissolving their membranes and subsequently increase the osteoinductive capacity of the DBP of the rat long and cranial bones. Allogenic DBP was prepared according to the methods described by Reddi and Huggins. Half of the DBP either the cranial and the long bone origins were incubated with Triton X-100. The other halves were used as control. Rats were divided into four groups [five each]. The DPB with different origins and treatment modalities was implanted between the rectus abdominus muscle and its fascia. Few DBP particles were processed for electron microscopy. The results showed that the amounts of newly formed bone in the cranial DBP implants treated in the control cranial DBP implants. The amounts of the newly formed bone in the cranial DBP implants in general was greater than that found in the long DBP implants. The osteoinductive capacity of the Triton X-100 treated cranial DBP is superior to the cranial DBP alone, long DBP treated with Triton X-100 and long DBP alone. The present study recommended the clinical use of Triton X-100 treated DBP in ridge augmentation and repair of facial defects