IL-1 alpha and il-8levels during gram-negative and gram-positive bacteremia in leukopenia leukemic patients

ABSTRACT

A quantitative relationships between circulating leukocytes and infection was established in patients with leukemia, in particular, the probability of being infected is proportional to the severity and duration of leukopenia [1]. Infection with gram- negative as well as gram-positive microorganisms may lead to septic shock and death [2, 3]. The major functional activities of cytokines are concerned with the regulation of the development and behavior of the immune effector cells. IL-1 is one of proximal cytokines. IL-1 act to stimulate the release of distal cytokines, such as IL-6 and IL-8, IL-8 is most closely related to the severity of the physiological response to infection and systemic inflammation. This study was conducted to detect the levels of IL-1 and IL-8 during Gram negative and Gram positive bacteremia. That could be useful in determining an appropriate choice for antimicrobial drug depending on the antimicrobial susceptibility pattern. IL-1 alpha and IL-8 was studied in [28] adult leukopenic patient with bacteremia, males and females, more than 15 years old. The study was including [20] healthy control. The bacteremic cases were obtained by culturing blood samples aerobically and anaerobically. The isolates were identified on the basis of their morphological, cultural and biochemical characteristics. Interleukin-1 alpha and interleukin-8 cocentration were measured by using a commercially available enzyme-linked immunosorbent assay [ELISA]. Statistical analysis show no significant difference in IL-1 alpha levels between Gram-negative and Grampositive bacteremia in leukopenic leukemia patients [P=0.803]. While statistical analysis show significant difference between patient with Gram-negative and Gram-positive bacteremia in leukopenic leukemia patients [P=0.037]. In leukopenic leukemia patients with bacteremia we may could determining an appropriate choice for treatment depending on IL-8 levels in the circulation of those patients and the antimicrobial susceptibility pattern