[Simultaneously determination of some drugs from different therapeutic groups in a biological and other medium by using the HPLC method]

ABSTRACT

The separation and determination of a selective drugs as fluoxetine, Oxybutynin, Valsartan, Enalapril, clonidine, ketoprofen [IS] were done in plasma by high-performance liquid chromatography reversed phase. The chromatographic conditions were gradient mobile phase A -Acetonitrile and B- 10mM KH[2]PO[4] buffer PH 3.3, flow rate 1.0 ml/min., column of octadecyle ODS YMC. 3 micro [150 mm x 46 mm] with guard column. The extraction method based on the technique used liquid/ liquid [LLE] and SEP. No interferences were between drugs and plasma in the mobile phase. The extraction recoveries were 86-90% for all products. This method permits the Simultaneous determination of all these compounds in plasma. Our method is simple and sensitive, based on qualitative and quantitative studies, the Coefficient regression was 0.9998 and Coefficient of variation was between 0.47-0.77% and the limit of detection was between 0.18-0.30 micro g/ L. The limit of quantification under the described conditions was 25-75 micro g/L for these drugs. Consequently, our results were useful for quality control in the therapeutic drug monitoring and the poisoning control centers and other fields