[Effects of nicotine on sperm motility in male mice under methylphenidate treatment]

ABSTRACT

Nicotine is an important part of cigarette smoke with strong physical and psychological effects on smokers. Methylphenidate [mph] is a medicine that has been prescribed widely for treatment of attention deficit hyperactivity disorder [ADHD]. In the present study, effects of both mph and nicotine on sperm motility which is one of the most effective factors in male fertility genital tract have been evaluated. In this experimental study, one hundred and twenty male mice divided in 11 treated and one control groups. In treated groups, mph with doses of 2 and 10 mg/kg and nicotine with doses of 100, 200 and 400 micro gr/kg were prescribed separately and in combinations orally for 40 days. At the final day, mice were killed and percent of sperm motility was calculated by counting gametes present in vasodeferan. Data were analyzed using Kruskal-wallis, Post-Hoc [Scheffe] and student's t-test. P<0.05 was considered significant. The sperm motility has been reduced significantly in groups that received both mph and nicotine. it can be concluded that even in light smokers, mph administration at high amount can reduce sperm motility