[Effects of harmane, norharmane and harmine on writhing behavior induced by acetic acid in mice]
Journal of Guilan University of Medical Sciences. 2011; 19 (76): 29-37
in Persian
| IMEMR
| ID: emr-110046
ABSTRACT
The beta-carbolines harmane, harmine and norharmane are the members of Harmala,s alkaloids group [Peganum harmala, Zygophillaceae]. The beta-carboline alkaloids adjoined to benzodiazepine site of the gamma-aminobutyric acid type A [GABA[A]]. These alkaloids also inhibited cyclooxygenase and lipoxygenase activities. These findings showed that the beta-carbolines should be able to reduce writhing nociception induced by acetic acid- in mice. To assess the effects of acute treatment with harmane, norharmane and harmine on the writhing induced by acetic acid in mice. The experiments were carried out on male BALB/C mice [20-25g]. Intraperitoneal [I.p] injection of acetic acid [0.6%] was performed in order to cause writhing behavior. This behavior was recorded by direct observation for a 30-minutes period. Decrease of writhing count is indicative of an anti-nociception. In order to avoid the possibility of a physicochemical interaction between them, Drugs were administered on opposite sides of peritoned. Intraperitoneal [I.p] injection of Harmane [5-20mg/kg] on 6-9 mice, norharmane [5-15mg/kg] on 8-9 mice and harmine [10-15mg/kg] on 8-9 mice in per group decreased the writhing behavior significantly [P<0.0001, P<0.0003 and P<0.0016, respectively]. The inhibitory effects of the mentioned drugs were antagonized by flumazenil [2 mg/kg]. Effects of harmane, norharmane and harmine on writhing response may be mediated through an inverse agonistic mechanism located in the benzodiazepine receptors
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Behavior, Animal
/
Pain Measurement
/
Treatment Outcome
/
Receptors, GABA-A
/
Pain Perception
/
Harmine
/
Acetates
/
Mice, Inbred BALB C
Limits:
Animals
Language:
Persian
Journal:
J. Guilan Univ. Med. Sci.
Year:
2011
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