[Differentiated dendritic cell with TGFbeta in reaction with TCD4+]

ABSTRACT

Dendritic cells are professional antigen presenting cells that obtain necessary regulatory signals for T cells. T regulatory cells are effective cells in reaction with dendritic cells and have an important role in treating of diseases. To evaluate the effect of TGF-beta [Transforming Growth Factor beta] Cytokine in dendritic cell generation and capacity of these cells in regulatory T cells development in culture media. In this experimental study blood samples were obtained from 5 volunteers then peripheral blood mononuclear cells were isolated by using Phicole Hypaque dendritic cells of peripheral blood monocyte were produced by GM-CSF IL-4 and TGF-beta in comparison with control group. Mixed leukocyte reaction was accesed in allogenic T cell and dendritic cell. The number of CD4+CD25+ T cells CD14+ and their surface markers were evaluated by Flowcytometry. In this study dendritic cells produced in presence and without presence of TGF-beta cytokine. The proliferation level of T cells decreased in mixed leukocyte reaction with dendritic cell-treated TGF-beta in comparison with control group. In addition the number of T CD25+ cells increased 27 percent in culture media including dendritic cell-treated TGF-beta in comparison with control group[p and amp;lt;0.05]. The TGF-Beta molecule caused effect on the dendritic cells and producted cells with inhibiting of immune responses in mixed leukocyte reaction. So it concluded that these cells can have an important role in preventing of unwanted immune reaction invitro