Some studies on the factors of the pathogenicity of Staphylococcus aureus isolated from bovine mastitis

ABSTRACT

This work aimed to study some factors of the pathogenicity of S. aureus isolated from bovine mastitis. Holstein-Friesian cows raised in two farms in El-Bohera governorate were examined from winter 2008 through spring 2009. Clinically, 208 out of 1000 cows had mastitis. Milk samples were collected and subjected for bacteriological examination for isolation and identification of S. aureus as well as further identification of S. aureus enterotoxin A [SEA] via PCR using species specific primers. Sixty-five [31.25%] isolates of staphylococci could be isolated. Twenty-two [33.85%] of the isolates were positive for coagulase activity. Two isolates out of eight [25%] biochemically identified S. aureus were positive for SEA. As detected by PCR