Association of total leukocytic count and differential leukocytic count as bioinflammatory markers with the glycemia of animal model of diabetes

ABSTRACT

To measure the White blood counts [TLC and DLC] and see their association with the glycaemic status [fasting blood glucose, glycosylated Hb] of normal and diabetic rats. This experimental study was carried out at Animal house / National Reference Laboratory for Poultry Diseases [NRLPD], Animal Division of National Agriculture Research Centre [NARC] Islamabad, Department of Biochemistry and Molecular Biology Army Medical College Rawalpindi from December 2006 to January 2007. A total of 60 albino rats [30 normal and 30 diabetic] were included in the study. Leucocytic counts were done by the visual method. Care was observed not to count clumped red cell debris. Blood glucose levels were determined by applying glucose oxidase method and the determination of total HbAlc was done using its Diagnostic kit. Significant difference was observed in the values of fasting glucose [p<0.05] but no significant difference was present in the values of Glycosylated Hb [HbAlc] [p>0.05] after experimentation. Comparison of total leukocytic count in normal and diabetic groups [p>0.05] revealed no significant difference. There was also no significant difference found in the values of Neutrophils and Lymphocytes counts [p>0.05]. Because of inadequate values of monocytes, Eosinophils and Basophils in both groups; the statistical analysis revealed no significant results. In a rat model of diabetes mellitus, hyperglycaemia of short duration has no role in producing inflammation as is indicated by no rise in the levels of otal leukocytic counts [TLC and DLC]