Chemical and biological studies of the methanolic extarct of primula elatior L. seeds

ABSTRACT

Fractionation and purification of the methanolic extract of the seeds of Primula elatior L [Primulaceae] cultivated in Egypt yielded six compounds named as beta-sitosterol-S-O-beta-D-glucoside [1], 4-ethyl gallic acid methyl ester [2], kaempferol [3], [+] catechin [4] together with the two polygalloylglucose derivatives, 1, 2, 3, 4, 6-penta-O-galloyl- beta-D-glucopyranose [5] and 1, 2, 3, 6-tetra-O-galloyl-beta-D-glucopyranose [6]. The structures of the isolated compounds were determined on the basis of extensive 1D [[1]H and [13]C] and 2D [COSY, HMQC, and HMBC] NMR studies, and mass spectral measurements. The anti-hypercholesterolemic effect of the total methanolic extract of Primula elatior L seeds has been studied and discussed