[ relationship between copper and zinc concentration and free radical markers in copper industry workers]

ABSTRACT

Copper and zinc play a key role in oxidative reactions and in vivo studies have shown their different effects. In this study we examined the influence of exposure to copper and zinc on catalase and superoxide dismutase [SOD] activities in erythrocytes, total serum antioxidant capacity and malondiadehyde [MDA] production of copper industry workers. In the present study, 70 workers in a Copper Industry and 70 healthy controls with the age range of 35-55 years were studied. The catalase assay and SOD activity in RBC lysate and total antioxidant were measured by spectrophotometric methods. Using thiobarbitoric acid reaction, MDA concentration was determined. The results showed a higher copper and zinc concentration in serum of worker group [113.8 +/- 2.2 and 148.3 +/- 3.2micro g/L respectively, P<0.001] in comparison with the control group [104.5 +/- 1.5 and 107.7 +/- 2micro g/L respectively]. Serum catalase and SOD activities in RBC were higher in workers [7621.7 +/- 199.3 and 1489.5 +/- 12.3micro /l] than in the control group [7049.1 +/- 157.4 and 1421.7 +/- 11.1 micro /l respectively, P<0.05, for SOD only]. Serum total antioxidant was significantly higher in workers than controls [1.6 +/- 0.03 vs. 1.4 +/- 0.01] and it was the same for MDA concentration [245.5 +/- 3.7nmol/gHb in the worker group compared to 205 +/- 3.2nmol/gHb in the control group]. No correlation were found between copper and zinc concentrations and the other factors [P>0.05]. These results suggest that occupational exposure to copper and zinc induces oxidative stress followed by anti oxidative defense system