Quantitative analysis of total proteins and carbohydrates in the digestive gland-gonad complex [DGG] and hemolymph of the freshwater prosobranch snail lanistes carinatus

ABSTRACT

Laboratory investigations were carried out to quantify the amount of total proteins, carbohydrates and reducing sugar in the hemolymph and Digestive Gland Gonad- complex [DGG] of infected and uninfected lanistes carinatus. Snails were naturally infected with two different types of trematode la val stages [rediae of gymnocepahalus cercaria and sporocyst of xiphidiocercaria], collected from the River Nile at Sohag governorate, Egypt. Analysis was carried out using an extraction of the DGGs tissue with buffer solution, while snails' hemolymph was applied directly. The results revealed that snail infection by rediae of gymnocephalus cercariae led to non significant increasing in both total carbohydrates and protein in hemolymph. However, infection by sporocysts of xiphidiocercariae caused a significant increasing only in hemolymph total protein. On the other hand, the amount of both reducing sugar and total proteins in DGG did not increase significantly whenever the infection caused by both types of trematode larvae. However, total carbohydrates in DGG increased significantly