[Toxicity Evaluation of coral madrepora and human BMG in peripheral mononuclear blood cells]

ABSTRACT

Disadvantage of auto grafts make widespread use of other bone graft materials such as xenografts and allografts. cytotoxic effect of materials and biomaterials on Lively human cells is the Object that always should be observed. In present study had tried to comparison evaluation cytotoxic effete of two bio material -human BMG and coral madrepora -on human peripheral mono nuclear blood cells. In present experimental study after to make preparation peripheral blood with fycole method and After preparation and sterilization of human BMG and coral madrepora, Then, human BMG and coral madrepora, aside to gether are divided into 10, 20, 50 mg portions and cultured on 24-well of 100000 mononuclear cells of peripheral blood and they are evaluated after 24, 48 and 72 hours. MTT method was used for evaluation of cytotoxic effect using ELISA reader in 540 nm wave lengths. Light absorption rate depends on MTT uptake by the cells, indicating vital activity of the cells. Result evaluated with variance and whilcoxon-rank sum and mann u whitnney test. All of the human BMG doses that were used weren,t toxic and all of them increased number of the human peripheral blood mononuclear cells [P< 0.005]. But coral madrepora, was decried number of the human peripheral blood mononuclear cells after 24 hour in 50 mg doses and after 48 hours in 10 mg doses and after 72 hours in 20 and 50 mg doses. With increasing of human BMG concentration and passing time, increasing of human peripheral blood mononuclear cells can be observed and it has a statistically significant difference with control group. Therefore, as a conclusion, human BMG is compatible with human blood mononuclear cells. But coral madrepora had toxicity for this blood cells. However for more judgment, need more study with different cells