Evaluation of chitosan/poly [vinyl alcohol] nano-composite biocompatibility for neural cells proliferation

ABSTRACT

Tissue engineering is an [interdisciplinary field that applies polymeric scaffolds to control tissue formation in three-dinemtion [3D]. The scaffold provides the microenvironment [synthetic temporary extracellular matrix] for regenerative cells, supporting cell attachment, proliferation, differentiation, and neo tissue genesis due to their suitable chemical, physical and biological structures. In this study, chitosan/poly [vinyl alcohol] [CS/PVA] was exploited as scaffold for nerve regeneration. Electrospinning was used to fabricate CS/PVA nanocomposites for U373 cells seeding and proliferation. Electrospinning is a versatile and simple method to fabricate non-woven thin layer fibers from polymeric solutions. Consequently, the biocompatibility of CS/PVA nanocomposite was evaluated using biological assays and cell attachment study. Results indicated that CS/PVA nanocomposites with 15/85 proportion shown an almost homogenous network of the electrospun fibers and confirmed that they can be knitted in meshes and improve U373 cells proliferation and cell attachment. The nano-sized CS/PVA scaffolds are nontoxic and biocompatible which can promote proliferation of U373 cells and their appropriate adhesion to nanocomposite for improved peripheral nerve regeneration