Effects of honey on carbon tetrachloride - induced kidney injury in adult male albino mice: light and electron microscopic study

ABSTRACT

In the recent years, extensive research work has been focused on the use of natural antioxidants against the toxic oxidative materials to ameliorate their toxic and cell damaging effects. In people who had died after exposure to CC14, kidney failure was frequently reported as the main cause of death. Honey is one of the natural antioxidants. So, the present work aimed at the morphological and morphometric evaluation of the efficacy of honey on the carbon tetrachloride-induced kidney injury.Thirty adult male albino Sprague-Dawley mice were divided into three groups, each group consisted of 10 mice. Control group [group I] received olive oil subcutaneously [S.C.]; CCL4 group [group II] were injected S.C. with 0.3% CCL4 dissolved in olive oil in a daily dose of 5ml/kg B. W. for 4 weeks. CCL4 + Honey group [group III] recieved both CCL4 and honey simultaneously daily for 4 weeks. The honey mixed with water and was given orally by gastric tube. The kidney specimens were processed for both light and electron microscopic examinations. Morphometric studies were also carried out. At L.M. level; carbon tetrachloride - induced degenerative changes in kidney cortex involved both corpuscles and tubules as well as inflammatory mononuclear cellular infiltration. By E.M.; thick interrupted glomerular basement membrane Morphometry showed significant changes of glomerulus, corpuscles and tubules. Honey effects were at L.M.; improvement of degenerative changes and inflammatory infiltration. At E.M; improvement of corpuscular and tubular changes. Morphometry showed better significant changes of glomerulus, corpuscles and tubules. Processed honey significantly reduced degenerative changes induced by CCL4 in the renal cortex on simultaneous administration