Effect of vitamins on growth criteria, photosynthetic pigments and some metabolic products of cobalte chloride stressed scenedesmus obliquus cultures

ABSTRACT

This study show the effect of applied vitamins ascorbic acid [vitamin C] and thiamine [vitamin B[1]] on growth criteria, total photosynthetic pigments total carbohydrate, total protein, free amino acids and proline contents of stressed Scenedesmus obliquus cultured for 7 days. The growth criteria [cell count and dry weight], total photosynthetic pigments, total carbohydrate contents, total protein contents, free amino acids and proline of stressed Scenedesmus obliquus cultures were significantly increased, when the algal cultures subjected to lower doses [1.5 and 3 ppm] of CoCI[2] only. On the other side, under relatively higher concentration [4.5 ppm] of CoGl[2] the cell count, dry weight, total photosynthetic pigments, total carbohydrate contents, total protein contents and free amino acids of CoCl[2] stressed Scenedesmus obliquus cultures were significantly decreased. However, the soluble carbohydrate contents, soluble proteins and praline contents of stressed Scenedesmus obliquus cultures were significantly increased. On the other hand, the parameters tested were significantly increased, when the algal cultures were subject to various doses [1.5, 3 and 4.5 ppm] of CoCI[2] and treated with 200 ppm applied vitamins [ascorbic acid or thiamine]. However, the different carbohydrate fractions [soluble, insoluble and total carbohydrate], soluble proteins of CoC1[2] stressed Scenedesmus obliquus cultures were significantly decreased