Effect of piroxicam and zinc on morphological and histochemical changes in mice liver

ABSTRACT

To observe the morphological and histochemical findings, produced by piroxicam and zinc in mice liver and correlate with serum hepatic enzyme. For this experimental study 30 adult mice [25 -30 grams] were obtained from animal house of Jinnah Postgraduate Medical Center, and divided into three groups i.e. A, B and C. Group A served as control and received normal diet, Group B received piroxicam 0.3 mg /Kg body weight intraperitoneally and Group C 1mg/Kg body weight of zinc intraperitoneally and piroxicam in the same dose as group B. After completion of study [6 weeks] animals were scarified and their livers were removed and after processing paraffin section were made and stained with Haematoxylin and Eosin for histological and histochemical examinations and correlate with serum hepatic enzyme level. Haematoxylin and Eosin stained section of group A indicated the normal histology and morphometry, the group B showed distorted hepatic lobular architecture. Central vein and sinusoids was dilated and congested, kupfer cell prominent and pyknotic cells and mono nuclear infiltration were seen. Group C showed altered histological findings comparable to group A. The Histochemical findings of group B showed depletion in glycogen content marked fibrosis of reticulin fibres and increased deposition of calcium phosphate crystals. In group C effect of zinc improve glycogen content and reticulin fiber deposition and decreased deposition of alkaline phosphatase crystals. The serum enzyme level of Alkaline Phosphatase and Serum Gulutamin Phosphatase significantly increased in group B animals and less significantly increase in group C as compared to group B animals. It was concluded that piroxicam in therapeutic dose, was toxic and produced hepatic injury and zinc along with piroxicam improved the hepatic damage