[Determination of fumaric acid in jelly using reversed phase high performance liquid chromatography [RP-HPLC]]

ABSTRACT

We can precisely determine the preservative Fumaric Acid in Jelly by using a reversed-phased HPLC method, that allows good separation after it is extracted by mean of dissolution. The separation was performed on C18 column within 10 min, by isocratic elution using water adjusted to pH 2.10-2.15 with perchloric acid, and detection was by UV absorption at a wavelength of 210 nm. Analytical characteristics of the separation such as limit of detection, limit of quantification, linear range and precision were evaluated. The developed method was applied for the determination Fumaric Acid in Jelly