Evaluation of the two FVIII assay types [clotting and chromogenic] for measuring FVIII activity in cryoprecipitate

ABSTRACT

In Iran, cryoprecipitate is an important plasma product for coagulation factors such as factor VIII [FVIII] to be ensured. As risk of bleeding is associated with FVIII activity, it is important to choose a suitable method for its measurement. In the present study we compared clotting and chromogenic assays on FVIII activity in cryoprecipitate. FVIII activity of cryoprecipitate from 48 donors with different blood groups was measured by clotting and chromogenic assays. Then the two assay types were compared with each other. Although the absolute values showed a significant difference, a good correlation was seen between one-stage clot-based and chromogenic assays for measuring FVIII activity in cryo. Although the one-stage clotting assay is a suitable method for measuring FVIII activity, the chromogenic assay has a good correlation with clotting based assay. Also a significant difference was seen between the two methods: