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P53 tumor suppressor gene evaluation in ovarian cancer by DGGE based mutation scanning
Ain-Shams Medical Journal. 2003; 54 (4,5,6): 383-391
in English | IMEMR | ID: emr-118316
ABSTRACT
Ovarian cancer remains the leading cause of death from gynecologic malignancy in western countries. This cancer results from a succession of genetic alterations involving oncogenes and tumor suppressor genes, which have a critical role in normal cell growth regulations. Forty-nine patients were studied for p53 mutations detection using combination of PCR amplification and DGGE which allows comprehensive mutation scanning for exons [5-8], mutation were confirmed by DNA gene sequencing. From 49 cases 5 cases showed mutation [10.2%] 3 mutations were detected in exon 6, one in exon 8, one in exon 5 [3] and all were point mutations. Of total mutations detected four were serous adenocarcinoma [4/24] [16.7%] and one was undifferentiated carcinoma. Detection of gene mutations by DGGE has proved to be a very accurate strategy
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Index: IMEMR (Eastern Mediterranean) Main subject: Polymerase Chain Reaction / Genes, p53 / Genes, Suppressor / Electrophoresis Limits: Female / Humans Language: English Journal: Ain-Shams Med. J. Year: 2003

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Index: IMEMR (Eastern Mediterranean) Main subject: Polymerase Chain Reaction / Genes, p53 / Genes, Suppressor / Electrophoresis Limits: Female / Humans Language: English Journal: Ain-Shams Med. J. Year: 2003