[Cloning and sequencing of L. major leishmania homologue of receptors for activated C kinase [LACK] gene]

ABSTRACT

Leishmaniasis is caused by parasitic protozoa of the genus Leishmania which in the infected host is obligate intracellular parasite. LACK gene is conserved among related Leishmania species. LACK is the immuno-dominant antigen of L.major which is considered as the most promising molecule for a recombinant or DNA vaccine against leishmaniasis. In this study the genomic DNA of an Iranian standard strain of Leishmania major [MRHO/IR/75/ER] was ertracted and the LACK gene was amplilified by PCR. Then the PCR product was cloned into pTZ57R/T cloning vector, The PT-LACK recombinant plasmid was extracted from transformed E.coli bacteria [TG1 strain] and sequenced. The LACK gene [Accession no LmjF28.2740] of MRHO/IR/75/ER and L. major was amplified using PCR method. LACK gene was cloned into pTZ57R/T coloning vector. Sequence analysis of the cloned LACK gene showed high homology 89% with LmjF28.2740 [LACK gene]. The LACK gene of L.major was cloned in pTZ57R/T vector successfully. Recombinant plasmid was confirmed and could be used for the production of recomiomort antigens is DNA vaccines, for further studies