[Evaluation of different Listeria monocytogenes components on dendritic cells for eliciting TH1 response]

ABSTRACT

Dendritic cells [DCs] play a critical role in the beginning and in the course of immune responses. By observing. Considering the defect in these cells in patients with tumor malignancy. In recent years there has been considerable interests in correction and use of these cells. In our prvious studies we showed that lysate of Listeria monocytogenese can induce maturation of dendritic cells, and induce TH1 response and increase the survival of tumor in an experimental model. The main objective in the present study is to evaluate the effects of different constituents of Listeria monocytogenes on DCs and their efficiency to induce TH1 response. After preparation of different components of Listeria monocytogenese [lysate of Listeria monocytogenes, protein and nucleic acid components], mouse bone marrow cells were cultured for 5 days in the presence of IL-4 and GM-CSF and treated with different components of Listeria for another 2 days. DCs were evaluated for the expression of Co-stimulatory molecules, MHC Class II molecules and Cytokine secretion. The results showed that, all of the components are able to mature DCs efficiently and induce TH1 response. But dendritic cells matured with protein components shift the response to TH1 more efficiently. Our findings indicated that dendritic cell maturation protein components of Listeria monocytogenese shift the response to TH1 more efficiently and may have beneficial effects in cancer immunotherapy