Improved spectrophotometric determination of disaccharides, hexoses and pentoses using aniline and its alkyl derivatives

ABSTRACT

Aniline and its alkyl derivatives in acetic acid media react with hexoses and pentoses to form colored products with characteristic absorption spectra. The spectral for individual hexoses, pentoses and their dehydration products and tend to vary with a given aniline derivative. The chromogen formed showed absorption at wavelength 530, 470 and 330mm. The method was improved and used to determine quantitatively glucoses, fructose, maltose, sucrose and ribose and the result obtained was found to obey Beer's law for a concentration range of [0.4-30mg mL-1] for glucose [0.2-2.2 mg.mL-1] for invert sugar [sucrose], [1-3,4mg.mL-1] for maltose under the specific condition that chosen for determination of each sugar