Impact of PGF2 alpha administration on progesterone pattern during and after PMSG treatment in buffaloes

ABSTRACT

Thirteen buffalo-cows and 6 heifers were subjected for superovulatory treatment using 3000 iu PMSG [Gestyl], 25 mg PGF2-alpha [Lutalyse] were injected 48 hours after PMSG treatment and at day eight of the estrous cycle after superovulatory treatments. Ovarian and progesterone changes were studied in buffalo-cows and heifers during and after superovulatory treatments. 84.6% and 83.3% of buffalo-cows and heifers exhibited estrous within 2.46 +/- 0.78 and 2.4 +/- 1.14 days, respectively, following treatment with lutalyse. Whereas, the intervals averaged 3.27 +/- 1.05 and 3.4 +/- 1.14 days when treated at day 8 after superovulation in buffalo-cows and heifers, respectively. Plasma progesterone concentrations averaged 3.74 +/- 1.53 and 1.28 +/- 0.91 ng/ml before treatment with lutalyse in buffalo-cows and heifers, respectively, followed by significant decline 24 hours after treatment in both buffalo-cows and heifers [1.12 +/- 0.71 and 0.74 +/- 0.38 ng/ml, p <0.01] and reached the lowest concentration 72 hours after treatment [0.20 +/- 0.09 and 0.24 +/- 0.24 ng/ml, respectively]. A marked elevation in plasma progesterone concentration following superovulatory treatment in buffalo-cows [15.02 +/- 10.44 ng/ml] and heifers [17.87 +/- 10.72 ng/ml] was recorded