Evaluation of the sensitivity of different methods for detection of seminal stains

ABSTRACT

identification of seminal stain by finding spermatozoa has long been regarded as the best and most certain means of detection. When the demonstration of complete spermatozoa was impossible, especially in persons having azoospermia as a result of disease or vasectomy, several methods have been sought for seminal fluid identification. The study showed that Florence test remained positive till five or six fold dilutions. On the other hand, Barberios and dithizone tests retained their positivity till 10-fold dilutions. As regards thin layer chromatography, the Rf value of fresh seminal stains was significantly higher than that of old seminal stains in undiluted specimen, but both could be identified till 164 dilution. A high creatine phosphokinase activity was reported with both fresh and old seminal stains, but it was significantly higher with fresh than with old stains till 116 dilutions. The activity of CK in fresh seminal stains remained high for less than a week [6 days]. This indicated that the presence of high CK activity might provide additional evidence for seminal fluid identification